The effect on the molecular concentration and electrical conductivity of muscle extracts of removal of the proteids / by G.N. Stewart.

  • Stewart, G. N. (George Neil), 1860-1930.
Date:
[1899?]
Catalogue details

Licence: Public Domain Mark

Credit: The effect on the molecular concentration and electrical conductivity of muscle extracts of removal of the proteids / by G.N. Stewart. Source: Wellcome Collection.

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    No. of Exp. 1 Frog’s muscle plasma, extracted by freezing and pressure +1 vol. water Another specimen of the plasma (not from the same frogs) Second specimen after removal of large precipitate of proteids coagulating at 30°—35° in bath After removal of the rest of the proteids by boiling \(5°)X108 82-84 48-19 78-97 85-10 91-20 3 4 5 Extract of dog’s muscle in 5°/0MgSO4 solution After dialysis for several days in running tap-water*, off globulin precipitate After further dialysis in distilled water for 2 days After further dialysis in distilled water for 8 days * Tap-water itself. 108-5 and filtering 15-77 14-64 54-75 f 1-64 t Nothing was added to the solution to prevent decomposition. Muscle extract in about 3°/0 MgS04 solution Dog’s thigh muscles. The MgS04 was added in substance after washing the muscle with water. •331 \cr0?c// ty- 72-49 VOlt L/cn> or, R OA .4 1-0092 After removal of 0*1132 grm. paramyosinogen from 100 c.c. •316 72-90 1-0163 1-1924 After removal of 0*1246 grm. myosin, from 100 c.c. •331 76-41 1-0158 1-2804 Muscle extract (made with 5°/0 MgS04 solution from leg muscles of dog) •509 113-00 1 -0271 2-3498 After removal of 0*1205 grm. paramyosinogen* from 100 c.c. •507 115-2 1-0266 2-3100 After removal of 0-1348 grm. myosin, from 100 c.c. •509 117-2 1-0263 2-3420 After removal of 0-0684 grm. myoglobulin and of 0 -0581 grm. of remaining coagulable proteids •508 117-0 1-0263 2-4012 The 5 °/o MgS04 solution •463 114-6 Total solids in 100 c.c. of original muscle extract 3-4056 grm. * The amount of paramyosinogen is probably a little too low. Muscle extract (made with 5 °/0 Mg S04 solution from thigh muscles of dog) •454 101-69 1 0244 1-7440 After removal of 0-2627 grm. paramyosinogen from 100 c.c. 1-0235 1-8240 After removal of 0*1079 grm. myosinogen •458 10124 1 0233 2-0094 After removal of 0-1079 grm. myoglobulin and 0-0954 grm. remaining coagulable proteid •482 103-17 1-0232 2-0525 The 5 °/0 MgS04 solution •433 104-32 1-0226 2-2812 Total solids in 100 c.c. of original muscle extract 3-0134.