Some new methods of test meal and feces examinations : their significance in clinical work / by Anthony Bassler.

  • Bassler, Anthony, 1874-
Date:
1910
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Licence: In copyright

Credit: Some new methods of test meal and feces examinations : their significance in clinical work / by Anthony Bassler. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    'between the two sides. The next step in the nutritive media test is to obtain samples of the important anaerobic bacteria. These are best ex- tracted by means of a pipette (b) which should be carefully introduced into the lower part of the anaerobic limb and about 0.5 c.c. of media sucked up. In tests to note the amounts of bacteria growing at different levels of the fluid media in the closed limb I have found that at the end of twenty-four hours most of the anaerobic bacteria are at the bottom of the tube, although they also are scattered throughout the closed limb and thus may be obtained from the upper layer of fluid when the cock has been opened. The carbon dioxide in the accumulated gases is now ab- sorbed by introducing into the closed limb with the pipette 2 c.c. of a saturated solution of sodium hydrate (making sodium carbonate) and the tube is inclined to permit this heavy solution to run some distance up into the closed portion and allowing it to stand for the time necessary to observe that the fluid no longer raises in the tube. In a number of tests to note the accuracy of this chemical method, I have observed that the remaining Co, is never more than from 5 to 10 per cent, of the original amount, and this is close enough for clinical work. The remaining mixed gases are then tested in the twin bottles, they being driven through when the cock is opened by means of attachment (e) and blowing. The reactions of the gases are noted in the first bottle (c), which contains a weak solution of neutral azolitmin (which is a pure litmus about 200 times as sensitive as commercial lit- mus), a control in a test tube being used to note the color change. In addition to the reaction of the gases, in the chemical and bacteriological