The constituents of cluytia similis / by Frank Tutin and Hubert W.B. Clewer.

  • Tutin, Frank.
Date:
[1912?]
Catalogue details

Licence: Public Domain Mark

Credit: The constituents of cluytia similis / by Frank Tutin and Hubert W.B. Clewer. Source: Wellcome Collection.

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    chlorine water and ammonia, thus indicating the presence of quinine or an allied alkaloid, the last-mentioned test was repeated, but with a negative result. Preliminary Extraction of the Plant.—A quantity (25 grams) of the dried and crushed material was extracted successively in a Soxhlet apparatus with various solvents, the following amounts of extract, dried at 100°, being obtained: Petroleum (b. p. 35 — 50°) extracted 0*83 gram = 3*32 per cent. Ether ,, Chloroform ,, Eth}7l acetate ,, Alcohol ,, Total 0-34 ,, = 136 0-22 ,, = 0-88 0-41 „ = 1-64 2-52 ,, =10*08 = 4*32 grams = 17 *28 per cent. For the purpose of a complete examination a quantity (7'42 kilo¬ grams) of the crushed and dried material was extracted by continu¬ ous percolation with hot alcohol, when, after the removal of the greater part of the solvent, 2‘17 kilograms of a dark-coloured extract were obtained. The entire amount of the above-mentioned extract was mixed with water, and steam passed through, the mixture for several hours, when a quantity (T24 grams) of an essential oil was removed. This oil possessed a pale yellow colour and a strong, rank odour. After the distillation of the extract with steam, as above described, there remained in the distillation flask a dark brown, aqueous liquid (A) and a quantity of dark green resin (B). The latter was separated, and repeatedly washed with boiling water, the concentrated washings being added to the main portion of the aqueous liquid. Examination of the Aqueous Liquid (A). The aqueous liquid (A) was concentrated somewhat, and extracted ten times with ether, the ethereal liquids being united, washed, and evaporated to a small bulk. A large volume of petroleum (b. p. 35—50°) was then added to the residue, after which the clear liquid was separated by decantation from the precipitated resinous material. The petroleum solution was then evaporated, the residue dissolved in ether, and the resulting solution shaken successively with aqueous ammonium and sodium carbonates and potassium hydroxide. Isolation of Chrysoyhanol, C15H10O4. The material soluble in ammonium carbonate was small in amount, and consisted chiefly of amorphous products. It also