The stimulating influence of serum on pancreatic amylase / by B.B. Crohn and A.A. Epstein.

  • Crohn, Burrill Bernard, 1884-1983.
Date:
1914
Catalogue details

Licence: In copyright

Credit: The stimulating influence of serum on pancreatic amylase / by B.B. Crohn and A.A. Epstein. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    Experiment 3. Comparison of sera from pathological conditions in their ability to stimulate the amylolytic activity of pancreatic amylase. A SERUM ALONE DILUTED 1:4, 0.5 CC. B PANCREATIC EXTRACT DILUTED 1:100, 0.5 cc. COMBINA- TION OF A AND B CC. cc. CC. Pneumonia 0.5 5 13 Diabetes mellitus (urine sugar-free j 0.5 5 12 Chronic nephritis 0.75 5 11 Uremia 0.5 4 13 Pleural fluid, pleurisy with effusion Pleural fluid (new growth of lung), non- 0.5 6 12 hemorrhagic Pleural fluid (new growth of lung), non- 0.5 6 13 hemorrhagic 0.5 6 13 In this experiment the addition of 0.5 cc. of diluted serum more than doubled the activity of pancreatic extract. The origin of the serum did not seem to play a role for the activating power of all specimens appeared to be the same, barring slight fluctuation due to possible imperfection in technique. Experiment 4. Activating effect of serum on human pancreatic secre- tion (duodenal contents, obtained by the Einhorn duodenal tube). A. Duodenal contents, normal case (dilution 1 : 10) 0.25 cc. used alone 3 cc. The same + human serum diluted 1 : 4, 0.25 cc. used 20 cc. B. Duodenal contents, cholelithiasis (dilution 1 : 10) 0.25 cc. used 6 cc. The same + human serum diluted 1 : 4, 0.25 cc. used 30 cc. this experiment shows marked activation of human pancreatic secretion by even a very small amount of human serum. Experiment 5. Effect of varying the quantity of serum used, on its activating power. In each tube, 0.5 cc. of pancreatic extract, dilution 1.. 100, having a constant digestive value of 5 cc. of 2 per cent starch solu- tion was used. CON- TROL A B c D E F Amount of serum added.... 2 per cent starch solution 0 AirCC. sVcc. TJP-C- vs cc. ice. 5CC. digested G 7 8.5 20 y io