The influence of an antihistamine drug on the release of histamine in the unanaesthetized dog / by W.D.M. Paton and M. Schachter.

  • Paton, William D. M.
Date:
[1951?]
Catalogue details

Licence: In copyright

Credit: The influence of an antihistamine drug on the release of histamine in the unanaesthetized dog / by W.D.M. Paton and M. Schachter. Source: Wellcome Collection.

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    TABLE I GASTRIC SECRETION IN RESPONSE TO COMPOUND 48/80 WITH AND WITHOUT MEPYRAMINE Compound 48/80, 10 mg. subcutaneously in all experiments. Italic figures refer to experiments in which 2 mg. mepyramine per kg. was given subcutaneously 15 min. before Compound 48/80 Dog c.c. 0.1n-HC1 secreted on days: 1 3 10 12 Ratio of secretion after 48/80 + mepyramine to secretion after 48/80 alone Spot .. 25 10 10 6 1.55 Dusky • • . . , . 60 124 106 14 3.11 Jackie . . 103 45 21 5 1.64 Blackie . . 32 37 40 19 1.52 Total 220 216 111 44 Av. 1.95 to stimulate secretion. The amount of saliva collected from the cannula over a period of 1-2 hours is not more than a few c.c., which did not interfere with the “ total ” hydrochloric acid titrations, since phenolphthalein was used as indicator. Regurgitation of bile is a rare occurrence under these conditions. Compound 48/80 was given as the chloride, propamidine as isethionate, mepyramine as maleate, and histamine as acid phosphate. All drugs were administered subcutaneously. The thickness of the jowl of the upper jaw about 1-2 in. from the snout was measured in duplicate with a micro¬ meter modified so that the jowl was enclosed between two flat surfaces during measure¬ ment. A lightly loaded spring ratchet enabled the reading to be taken with a repro¬ ducible gentle pressure, so that the maximum error with repeated measurement was 0.5 mm. In the experiments of Table II, the tests on each pair of dogs were crossed over, as indicated. Without Mepyramine With Mepyramine 20 Gastric Secret ion ccs. n/|0 HCI10 o • o Jowl thickness (0/0 X increase) y Time (min ) (a) O lO 30 50 70 90 Time (min ) W Fig. 1.—Dog Jackie. Gastric secretion (c.c. 0.1n-HC1 per 15 min. sample: upper curves), and increase in jowl thickness (%: lower curves), in response (a) to 10 mg. Compound 48/80 subcutaneously at zero time, (b) to 10 mg. Compound 48/80 subcutaneously at zero time, with mepyramine 2 mg./kg. subcutaneously 15 min. previously, (a) and (b) are each the average of Iwj crossed-over tests.