Cytoskeletal dynamics

Rendered 3D image showing part of an early chick embryo used to study cytoskeletal dynamics during development. The nuclei (pseudo-coloured yellow) were stained with DAPI and the actin (pseudocoloured purple) was stained with rhodamine phalloidin. Actin is a highly conserved protein found in all eukayotic cells and makes up the cytoskeleton. Imaging was carried out on whole embryos using a Biorad Radiance 2100MP multiphoton microscope. Technique: multi-photon fluorescence microscopy.