Human chromosome methodology / edited by Jorge J. Yunis.

Date:
1965
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Human chromosome methodology / edited by Jorge J. Yunis. Source: Wellcome Collection.

    13/280 (page IX)
    Contents Preface . vii Introduction xiii Sex Chromatin Techniques Murray L. Barr I. Introduction 1 П. Historical Sketch 2 III. Morphological Characteristics of the Sex Chromatin 3 IV. Sex Chromatin Techniques 5 V. Origin of the Sex Chromatin 13 VI. Sex Chromatin Patterns and Sex Chromosome Complexes 14 References 16 Human Peripheral Blood Leucocyte Cultures William J. Me I Iman I. General 22 II. The Mononuclear Leucocyte as the Mitotic Cell in Culture 23 III. Collection of Peripheral Blood Leucocytes and Preparation of Cell Inoculum 26 IV. Initiation of Mitosis in Blood Cultures: The Role of Phytohemag- glutinin and Other Potentially Mitogenic Agents 33 V. Influence of Culture Conditions on Cell Proliferation 36 VI. Preparation of Metaphase Spreads for Cytogenic Studies . 38 VII. Life Span of PHA Initiated Cultures: Evidence for Secondary Mitoses in Culture 40 VIII. Further Application of the Leucocyte Culture System . 4l IX. Methods for Culturing Human Peripheral Blood Leucocytes 42 References 46 Direct Chromosome Preparations of Bone Marrow Cells /. H. Tjio and J. Whang I. Introduction 51 II. Procedures 52 III. Discussion 53 References 56 ix \