The Marcus Beck Laboratory reports.

Date:
1914
Catalogue details

Licence: In copyright

Credit: The Marcus Beck Laboratory reports. Source: Wellcome Collection.

    16/86 (page 14)
    hours after they had been transferred into culture medium. The growth of human and other mammalian tissue was much slower than that of chick embryo tissue. In the latter, definite growth took place in twenty- four hours, and it continued to grow for three to five days, after which time the growth ceased and degenerative changes occurred, unless it was transplanted into fresh medium. With human and other mammalian tissue, on the other hand, no very definite growth occurred until about the fifth day, and this continued to increase till about the twelfth or thirteenth day, when it ceased and began to degenerate. We have not attempted so far to transplant growing human tissue into fresh media to prolong its life. (VI) SUMMAEY AND CONCLUSIONS. {a) Growth of human tumour tissue and normal human tissue can be obtained in vitro in a medium composed of fowl plasma to which extract of the human tissue, or extract of chick embryo, has been added. {h) The growth of epithelial cells differs from that of connective tissue cells. The former grow out in the form of buds or in a solid, advancing mass, whereas the latter radiate out in long, branching lines. (c) The new growing cells are much larger than the original cells from which they grow. They also contain numerous highly retractile granules and are markedly amoeboid, and tend to throw out long, proto- plasmic processes into the medium. {cl) The growing tissue tends to liquefy the medium, more especially the solid outgrowth of epithelial cell. ie) Connective tissue would appear to grow more readily than specialized epithelial tissue. if) The growth of human and other mammalian tissue in vitro is much slower than that of embryonic chick tissue in vitro, but the former tissues continue to proliferate in the original medium for a longer time before degeneration occurs. BIBLIOGRAPHY. Habrison, R. G. Journ. Exper. Zool, 1910, ix, p. 787.