A laboratory guide in elementary bacteriology / by William Dodge Frost.

Date:
1903
Catalogue details

Licence: In copyright

Credit: A laboratory guide in elementary bacteriology / by William Dodge Frost. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    List of Apparatus X Laboratory Rules XI List of References XII PART I.—GENERAL BACTERIOLOGY Chapter I. Morphology and Elementary Technique. EXERCISE PAGE 1. Cleaning Glassware 2 2. Plugging Flasks and Tubes 4 3. Sterilization of Glassware 4 4. Preparation of Bouillon... 6 5. Filling Test-tubes and Flasks with Culture Me- dia 10 6. Sterilization of Culture Media 10 7. Preparation of Gelatin... 14 8. Preparation of Agar 16 9. Preparation of Potatoes.. 18 10. Preparation of Water- blanks 18 11. Care of Culture Media.... 18 12. Platinum Needles 20 13. Test-tube Cultures 22 14. Incubation of Cultures.... 24 15. Study of Test-tube Cul- tures 26 16. Cleaning Slides and Cover- glasses 26 17. Preparation of Staining Solution 28 18. Simple Cover-glass Prep- arations 30 EXERCISE PAGE 19. Use of Microscope 34 20. Drawing Bacteria 36 21. Hanging-drop Prepara- tion 38 22. Microscopical Study of Form Types 40 23. Study of Cell Grouping... 42 24. Study of Involution Forms 46 25. Study of Endospores 46 26. Flagella Stain 48 27. Capsule Stain.. 50 28. Stain for Metaebromatic Granules 50 29. Morphology of Yeasts and Moulds Compared with Bacteria 50 30. Gelatin Plate Cultures.... 52 31. Agar Plate Cultures 56 32. Roll Cultures 56 33. Study of Plate Cultures... 58 34. Use of Decolorizing Agents 60 35. Gram’s Stain 60 36. Tubercle Stain (Gabbett) 62 Chapter II. Physiology op Bacteria. EXERCISE PAGE 37. Preparation of Special Media 64 38. Effect of Reaction of Me- dia on Growth 66 39. Effect of Concentration of Media on Growth 66 EXERCISE PAGE 40. Effect of Temp erature Variations on Rate of Growth 68 41. Determination of Thermal Death Point 68