Volume 3

Gene expression / Benjamin Lewin.

  • Lewin, Benjamin.
Date:
1974-1977
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Gene expression / Benjamin Lewin. Source: Wellcome Collection.

    13/952 (page IX)
    CONTENTS 1 Transformation 1 Genetic Transfer by DNA Molecules 1 discovery of transformation and the transforming principle .... 1 kinetics of transformation 6 Entry and Integration of Donor DNA 15 competence of bacterial populations 15 uptake of DNA 24 conversion of duplex DNA to single strands 27 integration of donor DNA 33 mutants defective in transformation 38 Genetic Mapping by Transformation 41 frequencies of transformation 41 efficiencies of integration 45 2 Conjugation 54 Nature of Bacterial Variation 54 identification of mutants 54 estimates for mutation rates 57 selection of mutants 60 Genetic Transfer between Bacteria 62 discovery of unidirectional genetic transfer 62 linkage between unselected and selected F+ markers 67 high frequency transfer by Hfr donors 71 efficiency of genetic transfer and recombination 79 surface properties of sexual mating types 81 Interactions between Sex Factor and Bacterial Chromosome .... 87 genetic transfer by different Hfr donors 87 insertion of F into the chromosome %