Drosophila development, endoplasmic reticulum movie

Visualisation of endoplasmic reticulum (e.r.) during Drosophila development, using the fluorescent lipophilic dicarbocyanine dye DiI. The animation shows the e.r. in a portion of the embryo starting at metaphase in cycle 10 and ending at late anaphase in cycle 14. During mitosis the signal is strongest at spindle poles, but no in the spindle itself. At telophase, there is accumulation of DiI at the midbody. During telophase the DiI signal becomes dispersed through the periplasm. As nuclei do not take up the dye, they remain as black areas with the reticulum moving around them.