Textbook of normal histology : including an account of the development of the tissues and of the organs.

  • Piersol, George A. (George Arthur)
Date:
1897
Catalogue details

Licence: Public Domain Mark

Credit: Textbook of normal histology : including an account of the development of the tissues and of the organs. Source: Wellcome Collection.

Provider: This material has been provided by the Augustus C. Long Health Sciences Library at Columbia University and Columbia University Libraries/Information Services, through the Medical Heritage Library. The original may be consulted at the the Augustus C. Long Health Sciences Library at Columbia University and Columbia University.

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    in distilled water to sunlight in a porcelain dish until it becomes dark brown ; the reduction is arrested, when sufficiently advanced, by thorough washing in water to which a few grains of sodium chloride have been added. The stained tissue may be mounted either in glycerin or in balsam, soaking in dilute and later strong glycerin, or dehydration and clearing, being the subsequent respective manipu- lations. Staining chromatin filaments for the display of karyokinetic figures and other studies of cell-structure can be successfully carried out only after accurate fixation of the cells, for which purpose the stronger Flemming's solution will be found most trustworthy. The tissue after such treatment is embedded in paraffin and cut, the fixed sections on the slide being subsequently stained by saffranin or by Delafield's haematoxylin. When karyomitosis is the especial object of study, preparations made by stripping off the epidermis of suitable animals {very young larval newts being excellent) are more favorable than sections, as the cells are preserved intact and contain the entire chromatin figures, and not merely the parts included within the planes of the section. Place small pieces of such tissues in— 3. Saffranin 2 gm. Alcohol, 50 per cent 60 c.c. 24-48 hours. b. Wash off in water for a few moments. c. Transfer to acidulated absolute alcohol {10 drops of pure hydro- chloric acid to 100 c.c. of absolute alcohol) for a few moments \TA—1 minute) until the clouds of color cease to be copiously given off; then— d. Transfer to fresh absolute alcohol for 1 to 2 minutes. e. Clear in clove oil and mount. Care must be taken not to remove too much color by prolonged action of either the acidulated or the plain absolute alcohol, since the preparation can be almost entirely bleached by inattention to this point. In a successful preparation the chromatin figures are brilliantly stained of a bright red, while the other parts of the cells are almost uncolored. Injection of capillary blood-vessels requires considerable ex- perience, and at best an element of uncertainty enters into every attempt, since the condition of the tissues, particularly of the vessels, largely influences the manner in which the fluid runs. While car- mine-gelatin injections make very attractive pictures, a successful blue mass possesses many advantages when used in connection with