Constancy of the optimum temperature of an enzyme under varying concentrations of substrate and of enzyme / by Arthur Compton.

  • Compton, Arthur.
Date:
1914
Catalogue details

Licence: In copyright

Credit: Constancy of the optimum temperature of an enzyme under varying concentrations of substrate and of enzyme / by Arthur Compton. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    of water, which, after standing from a half to one hour, weie introduced in portions of 1 cm.3 into four series of test-tubes containing 90 mgrm. of maltose dissolved in 4 cm.3 of water. The concentration of the substrate in this experiment is M/20, while the enzyme concentration varies between 2 x lO-4 and 20 x 10~4 grm. per cm.3. After 16 hours’ incubation, the action was stopped, and the quantity of maltose hydrolysed in each tube was determined as before. The numbers obtained are set out in Table III. On plotting the percentage of maltose hydrolysed against the mean tem- perature of the experiment the curves of fig. 2 are obtained. Here, again, each curve shows a maximum in the same region of temperature, + 47°. Consequently, the optimum temperature of the enzyme is independent of the enzyme concentration. Thus it is found, for the maltase of Aspergillus oryzce—as for the salicinase of sweet almonds—that the optimum temperature of the ferment is indepen- dent alike of the concentration of the substrate and of the concentration of the enzyme. II AliRISON and Sons, Printers in Ordinary to His Majesty, St. Martin’s Lane.