The student's manual of yeast culture / by Grove Johnson.

  • Johnson, Grove.
Date:
[1908?]
Catalogue details

Licence: In copyright

Credit: The student's manual of yeast culture / by Grove Johnson. Source: Wellcome Collection.

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    glass by a pair of forceps, pass it through the flame, and when cool mark upon it a droplet with the etching pen that has been dipped in the wort containing yeast cells. On the same side of the glass that has been marked with the droplet, paint the edge with vaseline. Now take the cavity glass, reverse it (it has been upside down until now), now place the cover glass in position, lift it a moment, breathe into the cavity so that the air be not too dry—the breath contains moisture—and now examine. If the operation has been successful the whole “ droplet ” will contain but a single yeast cell. Should the droplet contain more than one cell the operation must be repeated. The one inch objective having revealed but one cell in the droplet, it should next be examined by a one-eighth inch objective in order that a better idea of the species of the cell isolated may be determined. It will be remembered that the media is “ nutri-