The optimum temperature of salicin hydrolysis by enzyme action is independent of the concentrations of substrate and enzyme / by Arthur Compton ; [communicated by Sir J.R. Bradford].

  • Compton, Arthur.
Date:
1914
Catalogue details

Licence: In copyright

Credit: The optimum temperature of salicin hydrolysis by enzyme action is independent of the concentrations of substrate and enzyme / by Arthur Compton ; [communicated by Sir J.R. Bradford]. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    parable among each other, are required. This can only be achieved by varying the concentration of the enzyme at the same time as that of the substrate, for, ceteris paribus, the extent of an enzyme action is, as shown by the activity curves of figs. 1 and 3, determined by the proportion of enzyme to substrate present in the reaction mixture. The case which constitutes the fifth stage of the inquiry will now be considered. Table V. Temperatures at the beginning and end of each experiment. Salicin hydrolysed per cent, with the following molecular con- centrations of the substrate and grm. of the enzyme per cm.®. M/5. 31 X 10-®. M/10. 13-5 X icr®. M/15. 8-7 X 10-®. M/30. 5-7 X 10-®. M/50. 6 X 10-5. O 8-0 17 -2 9-0- 8-3 — 17 -3 9 -5- 9 -0 — — 18 -8 17 -8-17-5 35 -0 18 T-18 -2 — 34 -2 18 -2-18 -4 — — — — 39 -7 18 -4 - — 41 -9 18 -7-18 -5 — 40-3 18 -8-18 -7 — 36 -3 23 -1-22 -5 46 -1 24 -9-26 T — 60 -5 26 -1-26 -2 — — 53-3 26 -2-26 -4 60-3 61 -1 27 -0 — 56 -9 29 -7 62-2 30 -0-30 -5 — — — 65-7 30 -5 — — — — 68 -6 69-4 31 -8-31 -6 — — 63 -7 33 -5-33 -8 — 69 -4 65 -8 33 -8 — — 70 -0 70 -0 34 -7-34 -8 — 71 -7 36 -6-35 -7 — — 65-8 36 -0 — 69-4 36 -6 — — 66 -3 39 -4-39 -9 — — 69 -7 41 -8-42 0 60-5 58 -3 42 -5-43 -0 — — — 56-3 43 0 — — — 53 -0 43 -6-43 -5 — — 51 -2 44 ‘0-44 '4 — — — 47 -0 47 -8-48 -0 — 43 -3 48 -0-48 -5 — 37 -7 48 -5 — — 37-7 — — 36 -4 49 -0-49 -5 — — — — 34 -1 50 -2 44-6 51 -0 — 35 -6 51 -7-51 -6 — — 30 -7 63 -5 — 22 -7 53 -6-53 -8 — — — — 22 -5 22 -4 54 -6 33 -6 57 •6-67 -4 23 -7