Working with animal chromosomes / Herbert C. Macgregor and Jennifer M. Varley ; chapter 7 contributed by Aimee Bakken, R. Stephen Hill.

  • Macgregor, Herbert C.
Date:
[1983], ©1983
Catalogue details

Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: Working with animal chromosomes / Herbert C. Macgregor and Jennifer M. Varley ; chapter 7 contributed by Aimee Bakken, R. Stephen Hill. Source: Wellcome Collection.

    11/280 (page VII)
    Contents Preface xiii CHAPTER 1 CHROMOSOME SIZE, NUMBER, AND SHAPE 1 References 10 CHAPTER 2 MITOTIC CHROMOSOMES 13 I. Principles of the splash technique 15 II. Preparation of human chromosomes from lymphocytes using the splash technique 19 A. Equipment and materials 19 B. Procedure 20 III. Variations on the human lymphocyte technique 24 A. Adjustments to the preparation of shdes 24 B. Other mammalian species and other tissue types 24 C. All mitotic stages 26 IV. Non-mammalian sources 26 A. Avian leucocytes 27 B. Snake leucocytes 28 C. Amphibian intestinal epithelium cells 29 D. Amphibian bone marrow cells 30 V. Mitotic chromosomes using the squash technique 32 A. Amphibian intestinal epithelium tissue 32 B. Avian feather pulp 33 C. Neuroblasts of dipteran larvae 34 VI. Making permanent preparations 36 References 36 vii