The effect of serum-sensitiveness and precipitin formation upon the efficacy of diphtheria toxoid and toxin-antitoxin mixtures in promoting antitoxin production / A.T. Glenny, Barbara E. Hopkins and Hilda Waddington.

  • Glenny, A. T.
Date:
[1925?]
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Licence: In copyright

Credit: The effect of serum-sensitiveness and precipitin formation upon the efficacy of diphtheria toxoid and toxin-antitoxin mixtures in promoting antitoxin production / A.T. Glenny, Barbara E. Hopkins and Hilda Waddington. Source: Wellcome Collection.

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    rabbit, G. 218, out of six showed a big increase in response to the third injection and three responded to a smaller extent to an injection of 5 c.c. of toxoid preceded by horse serum than to the previous injection of 1 c.c. not preceded by serum. In the third group however the injection of toxoid in July, not preceded by horse serum, produced an increased response in all four rabbits. Similar results are seen when the third and fourth injections are compared. In groups 1 and 2, horse serum was not given before the September injection and an increased response was produced in all six rabbits while the inclusion of horse serum in group 3 caused two rabbits to show a lessened response while the remaining two remained at the same value. Table V. Showing the antitoxic value of the serum of ten imm,une rabbits after each of four injections of diphtheria toxoid preceded on alternate occasions by an injection of normal horse serum. Date of injection. April. June. July. September. Toxoid injected . (same for all 3 groups) 1*0 c.c. l'O C.C. 5*0 c.c. 5-0 C.C. Group 1.—Rabbits serum-sensitive at start. Normal horse serum. Rabbit 217 ,, 218 „ 222 ,, 225 5‘0 c.c. 2 ‘5 units 1*0 „ 0-2 „ 0-2 „ nil 5 •5 units 1-75 „ 1-75 „ 1-25 „ 5’0 c.c. 4*5 units 4'0 „ 2-0 „ 0-8 „ nil 7‘0 units io-o „ 5-0 „ 5-0 „ Group 2.—Control rabbits not previously serum-sensitive. Normal horse serum . Rabbit 215 „ 221 5’0 c.c. (2*5) (a) units (2-5) (a) „ nil 1 *0 units 5’5 „ 5*0 c.c. 1 ‘5 (b) units 3-5 (b) „ nil 5 '0 units 8-0 „ (a) Rabbits not serum-sensitive therefore figures not comparable with group 1. (b) Rabbits now serum-sensitive from injection of serum in April and figures now comparable with group 1. Group 3.—Rabbits serum-sensitive at start. Normal horse serum . Rabbit 216 „ 219 „ 223 „ 224 nil 1 ’5 units 0-8 „ 1*5 „ 1-5 „ 5*0 c.c. 0-9 units 1-25 „ 2.5 „ 3-5 „ nil 2*5 units 5-0 „ 7-0 „ 12-0 „ 5*0 c.c. 2*5 units 3*5 „ 7*0 „ 3*5 „ Summarising the results, we find that on all fourteen occasions the response to an injection not preceded by horse serum (in sensitised rabbits) was greater than the response to the previous injection preceded by horse serum, while six out of fourteen injections preceded