A hydrolytic derivative of the globulin edestin and its relation to Weyl's albuminate and the histon group.

  • Osborne, Thomas B. (Thomas Burr), 1859-1929.
Date:
[1900]
Catalogue details

Licence: In copyright

Credit: A hydrolytic derivative of the globulin edestin and its relation to Weyl's albuminate and the histon group. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    dantly diluted, yields no precipitate within several hours. Such a solution of edestan chloride yields a precipitate with io per cent, ammonia, which is soluble in a considerable excess, the resulting solution being readily precipitated by ammonium chloride. By decinormal ammonia solution the substance is precipitated but not redissolved, even by a very large excess of this ammonia solution. The aqueous solution of edestan chloride yields a precipitate with a very little ammonium or sodium chloride, the latter being readily, the former with difficulty, dissolved by an excess of ammonia. With nitric acid edestan gives a precipitate completely soluble on warming but reprecipitated on cooling. With potassium phosphotungstate, sodium phosphomolybdate or sodium picrate, edestan chloride forms slimy precipitates. With a solution of ovalbumin edestan chloride yields an abun- dant precipitate. Mercuric chloride forms no precipitate in a dilute solu- tion of edestan chloride, nor in a relatively strong one, unless a considerable amount of this reagent is added. Very dilute hydrochloric acid does not precipitate edestan, but the strong acid gives a precipitate, which dissolves in a suffi- cient excess of concentrated acid. e. The amount of acid with which edestan combines. Edestan exists in preparations of edestin chlorides in com- bination with acid. The amount of acid with which this sub- stance forms a compound, sparingly soluble in water, appears to be definite, as the following experiments show. A quantity of a preparation of edestin chloride was dis- solved, as far as possible, in io per cent, salt solution and the insoluble edestan was allowed to settle. This was first washed thoroughly with salt solution, until the edestin was removed, and then with water, in which a very little dissolved, while the remainder formed a gelatinous mass. The latter was sus- pended in water and dialyzed until the sodium chloride was removed. The dialyzer then contained an opalescent fluid and a voluminous precipitate. Of this fluid, 25 cc. were made neu- tral to phenolphthalein by adding 2.5 cc. of a centinormal solu-