A precise investigation of some micro-organisms and soluble ferments, their chemical history and relation to disease : including also a practical study of the disinfecting value of 'Sanitas' fluids / by C.T. Kingzett.

  • Kingzett, Charles Thomas, 1852-1935.
Date:
1885
Catalogue details

Licence: Public Domain Mark

Credit: A precise investigation of some micro-organisms and soluble ferments, their chemical history and relation to disease : including also a practical study of the disinfecting value of 'Sanitas' fluids / by C.T. Kingzett. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

    10/32 (page 10)
    This result is interesting because it proves that in addition to its property of preventing the development of yeast cells in glucose solution, together with the resulting chemical changes, Sanitas fluid also arrests their development instantly, even when in the- most active stage of growth. Experiment III.—A further solution of glucose, impreg- nated with yeast, was prepared, yielding a total solid residue of 8-016 per cent, dry at 100° C. Three mixtures of this solution were prepared as follows :— A. 75cc. of inoculated glucose solution + 25cc. water. B. 75cc. „ „ „ + 5cc. „ +20cc. Sanitas fluid. C. 75cc. of inoculated glucose solution + 25cc. of a 20 °/a solution of Sanitas emulsion.* The Sanitas fluid which was used in mixture B yielded 4-758 per cent, of solid matters, dry at 100° C. After standing seven days the experiments were brought to a conclusion by a chemical examination of each mixture and the following tabulated results were obtained :— Original Final Loss Per Cent. Alcohol Alcohol total solid contents, solid contents, in grins. of loss, formed, calculated. A. 6-012 4-2855 1-7265 28-55 0-870 0-882- B. 6-9636 6-9585 0-0051 0 073 None — C. 7-3355 7-287 0-0485 0-633 None — These results are confirmative of those observed in the earlier experiments, and at the same time show that very small quantities of Sanitas emulsion (and Sanitas oil, from which it is made) act like Sanitas fluid in preventing alcoholic fermentation de- pendent upon the growth of yeast. Yeast and the Fermentation of Starch. I thought it would be of some interest and importance to ascertain, if, by the living presence of yeast in a solution of starch, that chemical substance is resolved into alcohol. Such a result, if established by experiment, would be of importance, not alone from a chemical and technical point of view, but also as bearing upon the *=5cc. of Sanitas emulsion in the undiluted state, and consequently about 2-5cc. of Sanitas oil.