The essentials of histology, descriptive and practical, for the use of students.

  • Sharpey-Schäfer, E. A. (Edward Albert), Sir, 1850-1935.
Date:
1902
Catalogue details

Licence: In copyright

Credit: The essentials of histology, descriptive and practical, for the use of students. Source: Wellcome Collection.

Provider: This material has been provided by Royal College of Physicians, London. The original may be consulted at Royal College of Physicians, London.

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    The addition of alcohol to the above is not indispensable, but favours penetration. The piece is then washed in water for some minutes, trans- feri'ed to alcohol, embedded in celloidin or paraffin, and sections are prepared and mounted in the ordinary way. p. Place several small pieces of the fresh tissue direct in 2 per cent, silver nitrate solution at 35° C. in the dark : a piece is taken out on the third and on each subsequent day until the eighth. The piece is linsed in distilled water for a minute or two and then immersed in the developing solution for twenty-four hour.s : after which pi'oceed as above. Tlie tissue should be placed on cotton wool in the silver solution. The central parts of the tissue are usually best, the superficial parts being often too dark. 31. Bielchoivsky's method for neurojihrils.—Place small pieces in 12 per cent, formaline for twenty-four hours : wash in distilled water: transfer to 2 per cent, nitrate of silver for from three to five days : again wash in distilled water. Then transfer to the following solution, viz. : 2 per cent, nitrate of silver 20 c.c, to which thiee di'ops of a 40 per cent, solution of caustic potash are added, and enough ammonia to cause the disappearance of the precipitate jDi'oduced. The pieces may be left in this solution for some hours or days. They are then transferred to 20 pei' cent, formaline solution for twenty-four hours. Sections may be cut by the freezing or paraffin methods. The sections can be toned on the .slide with dilute gold chloride and afterwai'ds fixed with hyposulphite of .soda.