The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee.

  • Lee, Arthur Bolles, 1849-1927.
Date:
1885
Catalogue details

Licence: Public Domain Mark

Credit: The microtomist's vade-mecum : a handbook of the methods of microscopic anatomy / by Arthur Bolles Lee. Source: Wellcome Collection.

Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.

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    necessary in order to obtain a thorougli stain, maceration of the tissues supervening. But alcoholic staining fluids have still other advantages; they are vastly more penetrating; with them alone is it possible to stain through chitinous in- teguments ; they stain more quickly; the stain as a rule is more precise; and, if it be desired to stain slowly, tissues may be left in them for days without hurt. There are a few exceptional cases in which aqueous staining media are admissible, and some in which they are preferable to alcoholic media. Thus Mayer finds that picro-carmine is less hurtful to tissues than other aqueous stains, and attri- butes the result to the picrin contained in the solution; and other workers have found that alum-carmine gives very good results when employed after alcohol. And in general with tissues that have been well hardened in chromic acid the action of aqueous fluids does not appear to be seriously hurt- ful. Mayer observes that with certain groups of animals it is necessary to allow the tissues to be reimbibed and swelled up with a watery fluid in order to obtain the requii-ed action of the colouring agent. Lang finds that Turbellaria can only be successfully stained by means of an aqueous solution of picro-carmine and eosin.